OptiPrep™

OptiPrep™ is a ready-made, sterile and endotoxin-tested solution of iodixanol. Suitable for the isolation of many different cell types from blood and tissue (e.g. monocytes, lymphocytes, granulocytes, platelets, dendritic cells, megakaryotic progenitor cells; liver non-parenchymal cells, islet of Langerhans cells, spermatozoa; plant protoplasts) as well as for the isolation of cell organelles (e.g. nuclei, mitochondria, peroxisomes, lysosomes, endosomal subfractions) and membrane fractions (e.g. Golgi membranes, endoplasmic reticulum, specific plasma membrane domains). Separation of viable from non-viable cells. Also suitable for self-generated gradients. Highly effective for purification of viruses and virus particles. Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins.
Cat. No. 1114542
Density1.320 +/- 0.001 g/mL
Endotoxin<1.0 EU/mL
Quantity 1 x 250 mL
Price:
€165.00
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Description

OptiPrep™ is a sterile endotoxin tested solution of 60% iodixanol in water with a density of 1.32 g/ml. Iodixanol was developed as an X-ray contrast medium an has therefore been subjected to rigorous clinical testing. Iodixanol is non-ionic, non-toxic to cells and metabolically inert. Iodixanol solutions can be made isoosmotic at all useful densities. Iodixanol solutions have low viscosity and osmolality. Actual endotoxin levels in each batch are usually measured at < 0.13 EU/ml.

The high density of OptiPrep™ facilitates the fractionation of cells by flotation from a dense load zone through either a continuous or discontinuous gradient or through a simple density barrier.

55 protocols available for cell isolation.

Improved resolution of cell organelles. Low viscosity, isoosmotic gradients provide rapid and efficient separation of the major organelles in preformed gradients.

62 protocols available for isolation of subcellular organelles and membranes.

OptiPrep™ is the ideal solution for virus purification. Virus purified using OptiPrep™ gradients shows infectivity:particle number ratios at least 100x those from CsCl gradients.

31 protocols available for virus purification.

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