Description
Enzyme Immunoassay for the Detection of Human Antibodies against Hantavirus DOB/HTN in Serum.
Download the Application Upgrade for Hantavirus Dobrava / Hantaan IgG / IgM ELISA
Hantavirus – Reservoir, Infection and Diseases
Hantaviruses are single-stranded enveloped RNA viruses in the Bunyaviridae family, are widespread and are strictly associated with their serotype-specific reservoir hosts. They cause hemorrhagic fever with renal syndrome (HFRS) in Europe and Asia and lead to hantavirus cardiopulmonary syndrome (HCPS) in America. The severity of the disease depends on the infecting virotype with case fatality rates from 0.1% - 10% for HFRS and 25-35% for HCPS (13). Natural reservoir hosts are rodents, the virus is transmitted to humans via aerosol derived from rodent urine, feces or saliva. HFRS is caused by Hantaan virus and the closely related Dobrava virus as well as Seoul type and Puumala virus. Hantavirus serotypes show the same geographical distribution pattern as their reservoir hosts. Hantaan virus (host: Apodemus agrarius) is endemic in Asia, Dobrava virus (hosts: different Apodemus sp.) is distributed in the Balkans, Central, Eastern and Southern Europe, the form of the disease ranges from moderate to severe. Puumala serotype (host: Myodes clareolus) is predominant in Scandinavia and Western Europe and causes a mild infection, also called Nephropathia epidemica (NE). In Germany more than 90% of Hantavirus infections are caused by Puumala Virus. People at high risk are farmers, soldiers, campers.
Application of the Test
The Hantavirus Dobrava/Hantaan (DOB/HTN) IgG/IgM- ELISA is suited for the detection of specific IgG and IgM antibodies against DOB/HTN in cases of suspected hemorrhagic fever with renal syndrome (HFRS).
Principle of the Test
The microtiter plate is coated with recombinant nucleo- capsid protein of Hantaan virus. For determination of IgM antibodies, patient sera must be incubated with rheumatoid-factor-IgG-absorbent before starting the test procedure in order to eliminate unspecific reactions caused by IgG antibodies or rheumatoid factor. During the incubation period specific antibodies against the recombinant Hantaan antigen are bound to the solid phase. After washing, the specific IgG and IgM antibodies are detected with peroxidase-conjugated secondary antibody. Addition of substrate solution results in a color reaction, which is proportional to the bound specific antibody content. The absorbance is then measured photometrically.
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