Description
Molecular Weight
20 000
Purification
Heparin affinity chromatography
Biological Activity
≤ 3 ng/ml for half maximum stimulation of cell proliferation with bovine aortic endothelial cells (BAE cells).
Reconstitution
Reconstitute in 100 μl (10 μg; 25 μg) or 1 ml (1 mg) dist. H2O, dilute further in PBS as required.
Storage
at 2-8°C (lyoph.)
at -20°C (reconstituted)
Reference
Thomas KA et al: Purification and characterization of acidic fibroblast growth factor from bovine brain. Proc Natl Acad Sci USA 81, 357- 361 (1984)
Böhlen P et al: Acidic fibroblast growth factor (FGF) from bovine brain; amino-terminal sequence and comparison with basic FGF. EMBO J 4, 1951-1965 (1985)
Gimenez-Gallego G et al: Brain-derived acidic fibroblast growth factor: Complete amino acid sequence and homologies. Science 230, 1385-1388 (1985)
Jaye M et al: Human endothelial cell growth factor: Cloning, nucleotide sequence and chromosome localization. Science 233, 541-545 (1986)
Mergia A et al: The genes for basic and acidic fibroblast growth factors are on different human chromosomes. Biochem Biophys Res Comm 138, 644-651 (1986)
Linemeyer D et al: Expression in E. coli of a chemically synthesized gene for biologically active, bovine acidic fibroblast growth factor. Biotechnology 5, 960-964 (1987)
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