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Freely soluble in water up to 80% (w/v). Suitable for the the isolation of many different cell types from blood and tissue (e.g. monocytes, lymphocytes, granulocytes, platelets, dendritic cells, megakaryotic progenitor cells; liver non-parenchymal cells, islet of Langerhans cells, spermatozoa; plant protoplasts) as well as for the isolation of cell organelles (e.g. nuclei, mitochondria, peroxisomes, lysosomes, endosomal subfractions) and membrane fractions (e.g. Golgi membranes, endoplasmic reticulum, specific plasma membrane domains). Separation of viable from non-viable cells. Also suitable for self-generated gradients. Highly effective for purification of viruses and virus particles. Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins.
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