C-terminal domain (20 amino acids: C-NYD IGA ALD TIQ YSK HPP PL) of human p62 protein, coupled to KLH. This peptide sequence is identical in human, monkey, bovine, mouse, and rat.
Reactive Polypeptide (Specificity)
Human 62 kD (p62) protein, is present in intracytoplasmic inclusions (e.g. hyaline bodies) of hepatocellular carcinoma. p62 protein (also described as ubiqutin-binding protein; sequestosome 1; SQSTM1) has been found in many tissues and cells, including lymphoid cells, serving probably a common cellular signal transduction mechanism (e.g. ubiquitin-associated degradation and autophagy). The antiserum stains also neurofibrillary tangles in the brain of patients suffering from Alzheimer’s disease.
- immunohistochemistry on frozen and paraffin* sections (*enhanced after microwave treatment)
- cytological material - immunoblotting
1: 100 – 1:200 for immunohistochemistry
1:1,000 – 1:2000 for Western blotting (ECL)
1 h at RT; extended with paraffin sections
100 μl; contains 0.09% sodium azide
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Stumptner C, Fuchsbichler A, Heid H, Zatloukal K, Denk H: Mallory Body - a disease-associated type of sequestosome. Hepatology 35, 1053-1062 (2002)
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Janig E, Stumptner C, Fuchsbichler A, Denk H, Zatloukal K: Interaction of stress proteins with misfolded keratins. Eur J Cell Biol 84, 329-339 (2005)
Kuusisto E, Kauppinen T, Alafuzoff I: Use of p62/SQSTM1 antibodies for neuropathological diagnosis. Neuropathology & Applied Neurobiology pp 1-12 (2007)
BjørkøyG, Lamark T, Pankiv S, Øvervatn A, Brech A, Johansen T: Monitoring autophagic degradation of p62/SQSTM1. Methods Enzymol 452, 181-197 (2009)
Aishima S, Fujita N, Mano Y, Iguchi T, Taketomi A, Maehara Y, Oda Y, Tsuneyoshi M: p62+ hyaline inclusions in intrahepatic cholangiocarcinoma associated with viral hepatitis or alcoholic liver disease. Am j Clin Pathol 134,457-465 (2010)
Itakura E, Mizushima N: p62 targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J Cell biol 192, 17-27 (2011)